Goals

Goal 1: Guide RNA design and selection for CRISPR/Cas-based gene editing and gene expression regulation in plants

Goal 2: Ribozyme-mediated gene expression regulation in plants

Goal 3: RNA virus vector-based whole plant delivery of RNA-based genome editing and gene expression regulators

Tasks

Task 1: Design and optimization of CRISPR/Cas-based gene editing and gene-expression regulators

Task 2: Design and optimization of ribozyme-based gene-expression regulators

Task 3: Design and optimization of RNAi-based gene-expression regulators

Task 4: Construction of viral vectors for RNA delivery

Task 5: Fine-tuning of RNA-based gene expression regulation